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文献和实验A Proteomics Approach to Identify Redox-Sensitive Proteins
-Rad). 10. CB-X Protein Assay kit (G-Biosciences). 11. Reduction buffer: 50 mM Tris–HCl, pH 8.0, 7 M urea, 2 M thiourea, 1% CHAPS, and 1 mM DTT. 12. 11 cm ReadyStrip IPG strips (pH 4–7) (Bio-Rad). 13. SDS-PAGE running buffer (10×): 250
Methods for the Detection of D-Amino-Acid Oxidase
al. (5 ) using bovine serum albumin as a standard. It was also determined using a Protein Assay Kit (Bio-Rad, Hercules, CA). D-Amino-acid oxidase activity is finally expressed as the amount of D-alanine oxidized per min per milligram of protein. Hog
, 28304) Prime-It II Random Primer Labeling Kit (Stratagene, 300385) [alpha-32P]dCTP (ICN) Salmon Sperm DNA (Stratagene, 201190) SDS solution, 20% w/v (Bio-Rad, 161-0418) Zeta-Probe Genomic Tested Blotting Membrane (nylon) (Bio-Rad, 162-0196) Gel Blot
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