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文献和实验Microbiology - Serial Dilution
1. An undiluted (stock) solution is termed the 100 dilution. This may be a chemical solution, or a suspension of bacteria or bacteriophage. 2. Typical diluions are 10-fold dilutions; each dilution is 1/10th the concentration of the previous
Limiting dilution assays (LDA) are designed to define an unknown frequency of effector cells in a population. LDA are dose-response assays that allow detection of an all-or-none (positive or negative) immunoresponse in each individual culture
Cloning by Limiting Dilution of Hybridoma
must be greater than 80% to continue. For each hybridoma cell line, calculate the dilutions to give 4 cells/ml, 2 cells/ml and 1 cell/ml in cloning medium. Label 50 ml tubes for each clone and dilution. Add medium to each tube
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