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文献和实验【共享】流式 Overview of Flow Cytometry
/ well for each of several plates. As previously discussed, positive cloning selections need to be performed at low flow rates (
Procedures for In Vitro Production of Bovine Embryos
of Stock 2 (sodium pyruvate) per aliquot of OMM Laminar flow hoodProcedure1. The day before the collection of ovaries, prepare and place at 4°C until use. a) Saline + Pen/Strep -add 1 aliquot (10 ml) of Stock 16: pen/strep to each liter of transport
Marcantonio Lab Protocol Manual
cold room. 3) Aliquot culture into 8 pre-chilled sterile 50 ml polypropylene tubes. Let stand on ice for 10 minutes. 4) Centrifuge cells for 7 minutes at 3000 rpm at 4 degrees. 5) Pour off the supernatant and gently resuspend each pellet in 10 ml
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