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文献和实验Procedures for In Vitro Production of Bovine Embryos
is a lot but some of these tubes of HEPES-TALP will be used later in the day.2. Add 10 ml Sp-TALP to a 15 ml conical tube. Tighten the cap and place in the incubator.3. Add 5 ml IVF-TALP to a 15 ml conical tube. Leave cap loose and place in the incubator
! If too little volume is used, it will become trapped in column. Microspin G-25 column: Lot#______________ Resuspend the resin in the column by vortexing gently Loosen the cap 1/4 turn and snap off bottom, place in 1.5 ml screwcap eppendorf
Derivation and Culture of Dopaminergic Neurons (from Midbrains of Rodents)
with holes (e.g., Moria MC17) Dissecting microscope (e.g., Leica MZ6 or Zeiss Stemi 2000) Curved scalpel blade 实验材料 Embryonic (E14) rats or embryonic (E13) mice 实验步骤 1. Preparing Matrix
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