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文献和实验Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans
cells using a femtosecond laser nanoscissor. Mech. Chem. Biosyst. 2, 17-25 (2005). 13. Rao, G.N., Kulkarni, S.S., Koushika, S.P., & Rau, K.R. In vivo nanosecond laser axotomy: cavitation dynamics and vesicle transport. Opt. Express. 16, 9884-9894
so that a 75 x 25 x 1 mm microscope glass slide will sit flush in the chamber. 7) Create a recess for an O-ring around the underside of the top and bottom plates such that they are spaced apart by 0.02". Insert the rubber O-rings. 8)
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