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文献和实验Synthesis of Oligodeoxynucleotides with 5′‐Caps Binding RNA Targets
for the synthesis of oligodeoxynucleotides with a short peptidyl substituent linked to the 5??O ?terminus through a phosphodiester bond are presented. The example given is a peptidyl cap consisting of the residues of L ?prolinol, glycine, and the acyl residue
Genomic DNA Extraction for Mapping
5.spin down 15 to 20 min (5000 rpm) and transfer 300 μ l of the supernatant to a new 96 well plate (E&K, 1ml, polypropylene) containing 300 μl isopropanol/well 6.mix and wait 5 min at RT (can wait much longer) 7.spin 30 to 45 min (5000 rpm). Pour
Dual- and Triple-Color Fluorospot
100 μl/well of the mixed mAb solution and incubate overnight at 4–8°C (seeNote 1). c. After coating, empty the plate and wash five times with sterile PBS (200 μl/well) to remove excess mAb. Block the plate for at least 30 min with 200 μl
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