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上海希言科学仪器有限公司
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文献和实验Dialysis (using dialysis tubing)
: 1.Wash 30 minutes at 60℃ in a large volume (1000x) of 2% sodium bicarbonate and 1 mM EDTA) 2.Rinse tubing thoroughly with distilled water. 3.Cool and store at 4℃ in either 1% formaldehyde, 1% sodium benzoate, or 0.1% sodium azide
Preparation of Dialysis tubing
1)Boil the tubing on a sitr plate (preferably in the hood)in a 4L volume of 2% (w/v)sodium bicarbonate and 1mM EDTA pH 8.0 2)Rise the tubing in distilled water thoroughly 3)Boil for 10 minutes in 1mM EDTA (pH 8.0) 4)Allow tubing to cool
Dialysis (using dialysis tubing)
. Spectrapor tubing comes either wet (ready to use) or dry. Prior to use of dry membranes: 1.Wash 30 minutes at 60℃ in a large volume (1000x) of 2% sodium bicarbonate and 1 mM EDTA) 2.Rinse tubing thoroughly with distilled water. 3.Cool and store at 4℃
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