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文献和实验STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
at 37 C, sonicate 30" high, freeze lN2, thaw. 4) spin RT 10'' 15k ss34, respin in fresh tube 10'' 15k ss34. 5) decant again to new tube, add 5ml 50% Ni-NTA resin, mix gently RT 30''. 6) spin, decant and wash resin 3x in 50ml UPB6.3 w
Mag-Bind® mRNA Protocol (Standard Protocol)
, homogenize the sample in the microfuge tube using a disposable microtube pestle (OBI Cat No. SS-1015-00). The sample volume should not exceed 10% of the volume of RNA-Solv ® Reagent used. 2) Cells Grown in Suspension Pellet cells
Standard Operating Procedures for T1-Phage Testing Assay
10g tryp, 5g yeast, 10 g NaCl, 15g Agar x3 200-250ml each tray 200 ml LB Broth + 1.6 g Agarose x3 4 liter = 16 Q-trays
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