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文献和实验Care and Handling of Laboratory Mice
of laboratory mice in experimental microbiology and biomedical research is indisputable, and their care and handling is a contributing factor to the quality of the science resulting from their use. This unit provides guidelines for practical housing, handling
Practical Tips for Handling RNA
. Follow immediately with a quick 1-2 second centrifugation and aspirate again. Aspiration can be done with a syringe needle or a drawn-out Pasteur pipette connected to a vacuum source with a trap. Alternatively, a drawn-out Pasteur pipette
RNA Collection Purification from fibrous tissue
or liquid nitrogen. Alternatively， sample can be homogenized by syringe and needle method.(Also with mortar in liquid nitrogen). Disrupt tissue with 500μl of Buffer LY(add 20μl beta-mercaptoethanol per 1ml Buffer LY before use). Add 300
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