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文献和实验High Efficiency Yeast Transformation (Gietz Long Protocol)
and remove the LiAc with a micropipette. 11. The basic "transformation mix" consists of: 240 µl PEG (50% w/v) 36 µl 1.0 M. LiAc 50 µl SS-DNA (2.0 mg/ml) X µl Plasmid DNA (0.1 - 10 µg) 34-X µl Sterile ddH2O 360 µl TOTAL Carefully add these ingredients
or cosmid DNA of interest into a 12 X 75 mm Falcon tube containing 2 ml of LB media supplemented with the appropriate antibiotic (typically ampicillin at 100 ug/ml) and incubate at 37deg C 8-10 hours with shaking at 250 rpm. Transfer the culture
High efficiency LiAc transformation
media and incubate with shaking overnight at 30o C. 2. Count o/n culture and inoculate 50 mls of warm YPED to a cell density of 5 x 106 /ml culture. Note: i) Dilute overnight cultures 10-1 or more in water. ii) Carefully place 10
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