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文献和实验. 4). 10) Place a sterile syringe filter into the air vent (Fig. 4). 11) Move the flow circuit into a humidified incubator at 37 °C, 5% CO2 . 12) Clamp the hard tubing into the roller pump head indicated for use with this type
Whatman paper-5-6pcs per gel as well as Zetaprobe nylon membrane to size of the gel. Rinse nylon membrane in water Transfer in the following sequence: 3mm filter paper(soaked in NaOH)-------Gel-----------------Nylon membrane (presoaked in H2
to Minicolumn Reload syringe with DNA sample which has been resuspended in low salt buffer (5, 10 or 20 ml). Attach the Elutip pre-filter to the column. Attach syringe to pre-filter and column and slowly pass all of the sample through the filter
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