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文献和实验Chick Chorioallantoic Membrane (CAM) Assay
Dickinson, Bedford, MA) was diluted to a final concentration of 10 mg/ml with DMEM and directly pipetted onto meshes. Nylon mesh with 250 μm2 openings were cut into 4 mm x 4 mm squares and autoclaved. In preparation for polymerization, meshes were placed
preservatives (see Hint #2 ). B. Use of the Dialysis Tubing 1. After washing the tubing (see Protocol ID#113 ), calculate the length of tubing necessary to contain the volume of your protein sample and cut the tubing to length, leaving an extra inch
Myosin Light Chain Preparation
the 8 liter and the 28 liter beakers. 5.Put ETOH in the cold room. 6.Refrigerate or equilibrate the DE52 to be used. 7.Find parts to and assemble the 5½ cm ID column. 8.Make sure there is plenty of cold water in the cold room. 9.Thaw 8 M urea. Solutions
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