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文献和实验Recommended Reagent Concentrations: Primers: 0.2 - 1.0 uM Nucleotides: 50 - 200 uM EACH dNTP Dimethyl sulphoxide (DMSO): 0 - 10% (v/v) Taq polymerase: 0.5 - 1.0 Units/50ul rxn Target DNA: 1 ng - 1 ug (NB: higher concn for total genomic DNA
Standard neutral agarose electrophoresis
Standard neutral agarose electrophoresis Standard agarose gels can be prepared using either TBE or TAE running buffers. You will need: Either 10 x TBE or 10 x TAE buffers 1 x TBE buffer - 89mM Tris, 89mM boric acid, 2mM EDTA, pH
Standard Operating Procedures for T1-Phage Testing Assay
I. Introduction: This assay uses a lawn of phage-susceptible E. coli (DH10B) embedded in a layer of agarose. This top agarose lays on a bed of standard LB agar. If phage is present in a clone streaked or stamped onto the top agarose
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