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文献和实验screws, 1 pair of tweezers, one 75 x 25 x 1 mm glass slide (or other desired cell surface material), and 2 surgical towels. 6) Place sterile towels into laminar flow hood. Place the flow circuit, flow chamber, 4 x 4-way stopcocks, 1 x 1-way
A Live Cell Micro-imaging Technique to Examine Platelet Calcium Signaling Dynamics Under Blood Flow
The platelet is a specialized adhesive cell that plays a key role in thrombus formation under both physiological and pathological blood flow conditions. Platelet adhesion and activation are dynamic processes associated with rapid
. Thus, these biochemical and flow cytometric methodologies can be used to analyse signal-induced changes in phosphatidylinositol bisphosphate levels, diacylglycerol and inositol triphosphate production and calcium in each B cell population.
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