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文献和实验The new opportunities of modern assays of molecular biology can only be exploited fully if the results can be accurately correlated to the tissue phenotype under investigation. This is a general problem of non-in situ techniques
Primer Design（Manual and Automated Primer Design for SEQ and PHY Gaps）
General Primer General Primer Design Guidelines Note the 5’-3’ direction of the contig. Locate primers 100 to 200 bp from the feature. Pick primer from ≥ 2x high quality sequence coverage
Marcantonio Lab Protocol Manual
because they do not bind to the silica matrix, as well as removing any salts or ethidium bromide. 11) Spin the tube in a Speed-Vac concentrator to remove the last traces of NEW WASH. 12) Resuspend pellet in 25 ul of DD-H2O (or any other low salt buffer such as TE
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