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上海希言科学仪器有限公司
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文献和实验Methylated CpG Island Amplification
% ETOH Agarose gel electrophoresis reagents Filter hybridization reagents: 96 pin replicator system (Nunc) Nylon membranes DNA hybridization solution (e.g.BLOTTO) Random-primed DNA labeling kit Wash solutions (Wash1 2xSSC,0.1%SDS; Wash
Methylated CpG Island Amplification
Prepare tubes containing 10 m l of 10 X PCR buffer, 100 pmol of RXMA24 (or RMCA24) primers, 15 Units of Taq DNA polymerase, 1.2 m l dNTP mix (25mM), 0 m l (RXMA) or 5 m l (RMCA) DMSO, H2 O to a total volume of 97 m l. Add 3 m l of ligation mixture
Incubate at 16 ° C for 3-16 hours. 3.1.3 PCR amplification Prepare tubes containing 10 m l of 10 X PCR buffer, 100 pmol of RXMA24 (or RMCA24) primers, 15 Units of Taq DNA polymerase, 1.2 m l dNTP mix (25mM), 0 m l (RXMA
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