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文献和实验到Buffer Reservior 及1号Buffer Vial,注意将液面达到Marker。 3. 2号位为装有约4ml DI H2O的Buffer Vial, 3号位为注入DI H2O的去盖1.5ml eppendorf管。 4. 用适量的TSR溶解测序样品(根据试剂盒的Protocol),变性,冰上剧冷后,转入专用0.5ml样品管,盖好septa。 5. 于Manual Ctrl中,执行Autosampler HomeX,Y axis和Autosampler
The PRIDE Proteomics Identifications Database: Data Submission, Query, and Dataset Comparison
engines employed, and the automatic or manual filtering of identifications following the initial automated search. The PRIDE project provides an infrastructure to solve these problems, including a generic, standards-based format that can be annotated
, immunization, purification and analysis of antibodies. Carthew Lab Manual (University of Pittsburg) is a repository of Drosophila protocols, including somatic mosaic analysis, mutagenesis and immunostaining. Cell Biology Laboratory Manual (Gustavus
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