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文献和实验The CE instrument is controlled by a PC. The instrument settings are defined on the PC in method files. Settings, such as temperature, autosampler vial positions, injection parameters, and rinse cycles, are defined in the steps
Regulative Development in Axolotl Embryos; Splitting the Heart Field
, but demonstrates that the cells, when prevented from communicating with each other, will go on to form two separate organs. This is a direct result of regulative development. Materials Axolotl embryos (stage 14-16) 1% agarose-coated operating dishes
到Buffer Reservior 及1号Buffer Vial,注意将液面达到Marker。 3. 2号位为装有约4ml DI H2O的Buffer Vial, 3号位为注入DI H2O的去盖1.5ml eppendorf管。 4. 用适量的TSR溶解测序样品(根据试剂盒的Protocol),变性,冰上剧冷后,转入专用0.5ml样品管,盖好septa。 5. 于Manual Ctrl中,执行Autosampler HomeX,Y axis和Autosampler
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