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文献和实验Transgenic and Knockout Mice PROTOCOL
, and cut the band of interest. 2) Purifiying the DNA fragment. Load about 200-300µg of the gel cut onto a GeneClean spin column(BIO 101'S GeneClean Spinkit)--usually a fragment will require 6-8 columns to take care of all the gel.
Marcantonio Lab Protocol Manual
during the termination reactions. Transparent tape should be applied over any wells that will not be needed, in order to provide a better seal against water leaking into the sample wells. The horizontal rows should be labelled A-T-G and C, while the vertical columns
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