【分享】一些国外实验室的分子生物学实验方法链接(转）
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http://www.cellbio.com/protocols.html
Methods and Protocols
This section lists online resources for cell and molecular biology research protocols. Most of the web sites focus on particular research techniques.
Web sites of reagent sources often supply specific protocols and technical information relevent to their products. In addition, many laboratory home pages include protocols used in the lab. You can find a particular lab using a search engine or browse through Current Research.
An excellent new site, Protocol Online, collects and organizes many of the protocols that are available on the Web into a coherent database.
Antibodies: From Design to Assay is a comprehensive guide that discusses designing antigens, synthesizing peptides, immunization, purification and analysis of antibodies.
Carthew Lab Manual (University of Pittsburg) is a repository of Drosophila protocols, including somatic mosaic analysis, mutagenesis and immunostaining.
Cell Biology Laboratory Manual (Gustavus Adolphus College) is a comprehensive resource for basic cell biology methods, including cell fractionation, electrophoresis, microscopy, cell culture and differentiation experiments.
Comprehensive Protocol Collection (Ambros Lab, Dartmouth) is an extensive collection of general molecular biology protocols (RNA, DNA, protein methods) and C. elegans protocols.
DNA Sequencing Lab Protocols (Whitehead Institute/MIT Center for Genome Research) contains protocols for DNA purification and sequencing methods and M13 library construction from cosmids, BAC's or P1's.
ExPASy Molecular Biology Server (Swiss Institute of Bioinformatics) has a nice section for Technical information on 2-D PAGE.
Frequently Asked Questions from the bionet.molbio.methds-reagnts newsgroup is assembled by Paul Hengen.
Herskowitz Lab Protocols (UCSF) is a large collection of methods for S. cerevisiae genetics, cell biology and biochemistry.
Jorgensen Lab Protocol Database (University of Utah) contains general C. elegans procedures, molecular biology, microscopy and EM protocols.
Melbourne Signal Transduction Group Protocols (University of Melbourne) is an enormous collection of biochemistry, molecular biology and cell biology protocols from the Bowtell, Faulkner-Jones and Brennan labs.
Microscopy Methods (House Ear Institute, Los Angeles) provides information on light microscopy and transmission electron microcopy, including protocols for cryosectioning, immunocytochemistry and other specimen preparation techniques.
Mitchison Lab Protocols (Harvard) includes protocols for antibody preparation, general cytoskeletal techniques and Xenopus oocyte methods.
Molecular Biology Protocols (NWFSC) provides protocols for DNA purification, transformation and sequencing, PCR, electrophoresis and homology searches and has an active techniques forum.
Molecular Biology Techniques Manual (University of Cape Town) contains detailed descriptions and protocols for PCR, SDS-PAGE & Western blotting.
PCR Jump Station (Horizon Press) is a nicely organized collection of links to PCR resources.
Promega Technical Bulletins and Manuals is a huge compendium of molecular biology protocols, including information on enzymes, vectors, sequencing, purification and expression systems.
Protocol Online (DoubleTwist, Longcheng Li, UCSF) is an excellent new site that collects and organizes many of the protocols that are available on the Web into a useful, coherent database.
Recombinant DNA Technology Course (University of Minnesota) is a simple flowchart that summarizes basic molecular biology techniques.
Schimenti Lab Technical Protocols (The Joy of Cloning) includes, DNA and RNA techniques and mouse genotyping methods.
General Informational Resources
The Antibody Resource Page features an extensive compilation of antibody information and advice for locating specific antibodies.
Biocompare.com catalogues and compares biological research products in an easy and efficient database.
LabConsumer (The Scientist) presents updated information about biological methods, research tools and reagents.
REBASE (The Restriction Enzyme Database) presents tons of information (published and unpublished) on restriction enzymes and methylases.
Technical Information (FMC BioProducts) discusses agarose and agarose gels in extensive detail.
Vector DB (Anderson Unicom Group, Inc.) is an extensive database of vectors commonly used in molecular biology.
Links to protocols
This site gives you general information on how and why molecular techniques work. Here are links to some sites that give you specific protocols for performing individual techniques.
BIOSCI. A set of electronic communication forums—the bionet USENET newsgroups and parallel e-mail lists—used by biological scientists worldwide. No fees are charged for the service.
http://www.bio.net/
Cell and Molecular Biology Online's methods and protocols page.
http://www.cellbio.com/protocols.html
Comprehensive protocol collection. From the Ambrose lab at Dartmouth College.
http://cobweb.dartmouth.edu/~ambros/molbio/index.html
http://www.uct.ac.za/depts/mmi/bbhelp/con2.html#CON_PRX_MAN
Hugh Griffin's site. This site will link you to a variety of molecular biology pages, including many protocols. This page has a large number of sponsor banners, so be prepared for it to load a little slower.
http://www.highveld.com/protocols.html
Mark Strom's Molecular Biology Protocols site. Mark Strom at the Microbial Pathogenesis/Utilization Research Division, Northwest Fisheries Science Center (NMFS/NOAA). Lots of protocols and helpful hints.
http://research.nwfsc.noaa.gov/protocols.html
Molecular Biology Techniques Manual. An excerpt from the hardcopy version. Includes descriptions and protocols for SDS-PAGE, PCR, and Western blotting. Lots of highly technical information about these techniques.
http://www.cellbio.com/protocols.html
PCR and multiplex PCR: guide and troubleshooting. An online PRC manual put together by Octavian Henegariu of Yale University.
http://info.med.yale.edu/genetics/ward/tavi/PCR.html
Protocol Online. A database that organizes many online protocol resources. This site also has discussion forums.
http://www.protocol-online.net/
Protocols for recombinant DNA isolation, cloning, and sequencing. An extensive online manual of everyday lab techniques. Put together by Bruce A. Roe, Judy S. Crabtree, and Akbar S. Khan, Department of Chemistry and Biochemistry, The University of Oklahoma, Norman.
http://dna1.chem.ou.edu/protocol_book/protocol_index.html
Protocols from the Crawford Laboratory at the University of Missouri, Kansas City.
http://sgi.bls.umkc.edu/funnylab/DLCprotocols.html
Technical Tips Online. A peer-reviewed site that includes protocols, handy tips, and product information.
http://tto.trends.com/tto/index_of_TTO.shtml
Methods and Protocols
This section lists online resources for cell and molecular biology research protocols. Most of the web sites focus on particular research techniques.
Web sites of reagent sources often supply specific protocols and technical information relevent to their products. In addition, many laboratory home pages include protocols used in the lab. You can find a particular lab using a search engine or browse through Current Research.
An excellent new site, Protocol Online, collects and organizes many of the protocols that are available on the Web into a coherent database.
Antibodies: From Design to Assay is a comprehensive guide that discusses designing antigens, synthesizing peptides, immunization, purification and analysis of antibodies.
Carthew Lab Manual (University of Pittsburg) is a repository of Drosophila protocols, including somatic mosaic analysis, mutagenesis and immunostaining.
Cell Biology Laboratory Manual (Gustavus Adolphus College) is a comprehensive resource for basic cell biology methods, including cell fractionation, electrophoresis, microscopy, cell culture and differentiation experiments.
Comprehensive Protocol Collection (Ambros Lab, Dartmouth) is an extensive collection of general molecular biology protocols (RNA, DNA, protein methods) and C. elegans protocols.
DNA Sequencing Lab Protocols (Whitehead Institute/MIT Center for Genome Research) contains protocols for DNA purification and sequencing methods and M13 library construction from cosmids, BAC's or P1's.
ExPASy Molecular Biology Server (Swiss Institute of Bioinformatics) has a nice section for Technical information on 2-D PAGE.
Frequently Asked Questions from the bionet.molbio.methds-reagnts newsgroup is assembled by Paul Hengen.
Herskowitz Lab Protocols (UCSF) is a large collection of methods for S. cerevisiae genetics, cell biology and biochemistry.
Jorgensen Lab Protocol Database (University of Utah) contains general C. elegans procedures, molecular biology, microscopy and EM protocols.
Melbourne Signal Transduction Group Protocols (University of Melbourne) is an enormous collection of biochemistry, molecular biology and cell biology protocols from the Bowtell, Faulkner-Jones and Brennan labs.
Microscopy Methods (House Ear Institute, Los Angeles) provides information on light microscopy and transmission electron microcopy, including protocols for cryosectioning, immunocytochemistry and other specimen preparation techniques.
Mitchison Lab Protocols (Harvard) includes protocols for antibody preparation, general cytoskeletal techniques and Xenopus oocyte methods.
Molecular Biology Protocols (NWFSC) provides protocols for DNA purification, transformation and sequencing, PCR, electrophoresis and homology searches and has an active techniques forum.
Molecular Biology Techniques Manual (University of Cape Town) contains detailed descriptions and protocols for PCR, SDS-PAGE & Western blotting.
PCR Jump Station (Horizon Press) is a nicely organized collection of links to PCR resources.
Promega Technical Bulletins and Manuals is a huge compendium of molecular biology protocols, including information on enzymes, vectors, sequencing, purification and expression systems.
Protocol Online (DoubleTwist, Longcheng Li, UCSF) is an excellent new site that collects and organizes many of the protocols that are available on the Web into a useful, coherent database.
Recombinant DNA Technology Course (University of Minnesota) is a simple flowchart that summarizes basic molecular biology techniques.
Schimenti Lab Technical Protocols (The Joy of Cloning) includes, DNA and RNA techniques and mouse genotyping methods.
General Informational Resources
The Antibody Resource Page features an extensive compilation of antibody information and advice for locating specific antibodies.
Biocompare.com catalogues and compares biological research products in an easy and efficient database.
LabConsumer (The Scientist) presents updated information about biological methods, research tools and reagents.
REBASE (The Restriction Enzyme Database) presents tons of information (published and unpublished) on restriction enzymes and methylases.
Technical Information (FMC BioProducts) discusses agarose and agarose gels in extensive detail.
Vector DB (Anderson Unicom Group, Inc.) is an extensive database of vectors commonly used in molecular biology.
Links to protocols
This site gives you general information on how and why molecular techniques work. Here are links to some sites that give you specific protocols for performing individual techniques.
BIOSCI. A set of electronic communication forums—the bionet USENET newsgroups and parallel e-mail lists—used by biological scientists worldwide. No fees are charged for the service.
http://www.bio.net/
Cell and Molecular Biology Online's methods and protocols page.
http://www.cellbio.com/protocols.html
Comprehensive protocol collection. From the Ambrose lab at Dartmouth College.
http://cobweb.dartmouth.edu/~ambros/molbio/index.html
http://www.uct.ac.za/depts/mmi/bbhelp/con2.html#CON_PRX_MAN
Hugh Griffin's site. This site will link you to a variety of molecular biology pages, including many protocols. This page has a large number of sponsor banners, so be prepared for it to load a little slower.
http://www.highveld.com/protocols.html
Mark Strom's Molecular Biology Protocols site. Mark Strom at the Microbial Pathogenesis/Utilization Research Division, Northwest Fisheries Science Center (NMFS/NOAA). Lots of protocols and helpful hints.
http://research.nwfsc.noaa.gov/protocols.html
Molecular Biology Techniques Manual. An excerpt from the hardcopy version. Includes descriptions and protocols for SDS-PAGE, PCR, and Western blotting. Lots of highly technical information about these techniques.
http://www.cellbio.com/protocols.html
PCR and multiplex PCR: guide and troubleshooting. An online PRC manual put together by Octavian Henegariu of Yale University.
http://info.med.yale.edu/genetics/ward/tavi/PCR.html
Protocol Online. A database that organizes many online protocol resources. This site also has discussion forums.
http://www.protocol-online.net/
Protocols for recombinant DNA isolation, cloning, and sequencing. An extensive online manual of everyday lab techniques. Put together by Bruce A. Roe, Judy S. Crabtree, and Akbar S. Khan, Department of Chemistry and Biochemistry, The University of Oklahoma, Norman.
http://dna1.chem.ou.edu/protocol_book/protocol_index.html
Protocols from the Crawford Laboratory at the University of Missouri, Kansas City.
http://sgi.bls.umkc.edu/funnylab/DLCprotocols.html
Technical Tips Online. A peer-reviewed site that includes protocols, handy tips, and product information.
http://tto.trends.com/tto/index_of_TTO.shtml
