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文献和实验Transformation of E. coli by Electroporation
-DNA mixture between the bosses in a Micro-Electroporation Chamber. Do not leave an air bubble in the droplet of cells; the pressure of a bubble may cause arcing and loss of the sample. Place the chamber in a slot in the Chamber Rack and note
UPDATED DIRECTIONS FOR NEW BRUNSWICK MOBILE PILOT PLANT FERMENTOR
It is important to always have seal steam pressure (10-15 psi) when stirring the media, even if the unit is not in sterilization mode. This is because the steam condensate is necessary to lubricate the seal. Make sure the seal steam pressure is up to the necessary
The partial pressure of oxygen (pO2 ) plays a determining role in the energy metabolism of aerobic cells. However, low pO2 level induces pathophysiological conditions such as tumor hypoxia, ischemia or reperfusion injury, and delayed/altered
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