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上海希言科学仪器有限公司
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文献和实验with a syringe. Using flat-tipped gel loading pipette tips, load each odd-numbered sample. Pre-electrophorese the gel for at least 5 minutes, flush the wells again, and then load each even-numbered sample. 9. Begin the electrophoresis (30 W for 10 hours) run
in each well is suctioned out with a mouth pipette.Each base-specific sequencing reaction terminated with the short termination mix is loaded using a mouth pipette onto a 0.15 mm X 50 cm X 20 cm, denaturing 5% polyacrylamide gel and electrophoresed for 2.25 hours at 22 mA
Attachment of Nitroxide Spin Labels to Nucleic Acids for EPR
carbonate (NaHCO 3 ; 5% w/w aqueous solution) MgSO 4 Silica gel (grade 60, 70 to 230 mesh, 60 Å; Merck; Sigma‐Aldrich
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