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上海希言科学仪器有限公司
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文献和实验. Briefly rinse corner of bag around injection site to remove any probe on outside of bag. Hybridize the filters at 65℃ in a slowly rotating H 2 O bath overnight.Day 2 Prepare in Advance: 5X SSPE, 65℃ (0.5 - 1L) 1-2 L 2x SSPE 1-2 L 1x SSPE Whatman 3MM
tube. Add 2 ml of homogenization buffer (4 ml per g) and disperse the tissue in it. 4) Leave for 10 min at room temperature. 5) Add 2 ml of phenol/chloroform and vortex. OR, 1) Put a small or medium sized leaf into a 4" x 6" 500 guage
into a 4" x 6" 500 guage (thick!) plastic bag. 2) Add 2-3 ml homogenization buffer (you may need more for large leaves) to the bag. 3) Grind the leaf inside the bag using the top end of a 50 ml corex tube. 4) Pour the homogenate immediately into a 15
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