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文献和实验with white inserts. Transfer supernatant to new 15 ml c/f tubes(2). Add 800 l chloroform to each tube. Vortex 15 sec, incubate at room temperature for 3 min. Smeary preps will result if vortexing not long enough. Spin at 4°C, 4000 rpm
1. Add appropriate volumes of RNA ( Try 5 and 10 ug RNA plus 600 or 800 pg dig labelled probe. 2. Include 2-3 yeast RNA samples/probe used. 2ul of 5mg/ml stock. 3. Add 20 ul of Soln. A to all of the tubes (F.V.= 30 ul) vortex
1. Add appropriate volumes of RNA ( Try 5 and 10 ug RNA plus 600 or 800 pg dig labelled probe. 2. Include 2-3 yeast RNA samples/probe used. 2ul of 5mg/ml stock. 3. Add 20 ul of Soln. A to all of the tubes (F.V.= 30 ul) vortex and spin
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