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文献和实验Dual-Color ELISPOT Assay for the Simultaneous Detection of IL-2 and/or IFN- Secreting T Cells
and is detected when the color of a spot meets the threshold at the intersection of both single colors. iii. Count the cells in each of the three types of antigen-specific cell populations: single IFN- responding cells, single IL
A SENSITIVE METHOD FOR DETECTION OF APOPTOSIS BY SINGLE LASER FLOW CYTOMETRY
with single or dual-color phenotype and the use of FITC and/or PE conjugated monoclonal antibodies. Surface antigen staining is done according to standard methods before staining for apoptosis. Then, the apoptosis assay is performed as described above.
Fluorescent Indicator Methods to Assay Functional CFTR Expression in Cells
of materials, are technically difficult, and cannot be used to study single cells or heterogeneous cell mixtures. Single-channel electrophysiological measurements of CFTR function are also technically challenging and generally not suitable for screening
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