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文献和实验Dual-Color ELISPOT Assay for the Simultaneous Detection of IL-2 and/or IFN- Secreting T Cells
and is detected when the color of a spot meets the threshold at the intersection of both single colors. iii. Count the cells in each of the three types of antigen-specific cell populations: single IFN- responding cells, single IL
Dual-Color ELISPOT Assay for the Simultaneous Detection of IL-2 and/or IFN- Secreting T Cells
and is detected when the color of a spot meets the threshold at the intersection of both single colors. iii. Count the cells in each of the three types of antigen-specific cell populations: single IFN- responding cells, single IL
A SENSITIVE METHOD FOR DETECTION OF APOPTOSIS BY SINGLE LASER FLOW CYTOMETRY
with single or dual-color phenotype and the use of FITC and/or PE conjugated monoclonal antibodies. Surface antigen staining is done according to standard methods before staining for apoptosis. Then, the apoptosis assay is performed as described above.
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