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上海希言科学仪器有限公司
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文献和实验Recombineering/Lambda red-mediated gene replacement
Transformation Buffer 10 mM Pipes 15 mM CaCl2 250 mM KCl Titrate to pH 6.7 before adding MnCl2 55 mM MnCl2 Filter sterilize Incubate on ice for 10 min
Hybridization of High Density Arrayed BAC Nylon Filter Blots
above into one 1.8ml microcentrifuge tube. Add 150µl Human Placental DNA (Sigma, 10.9mg/ml, D-3287). Add 250µl Modified Hybe Solution: Reagent Final NaCl 1M Tris pH8.0 0.05M EDTA 5mM SDS 1% Dextran Sulfate 10% Boil, 8min
HIGH RESOLUTION GENETIC FOOTPRINTING
stock solutions of Tris, EDTA, NaCl, Glycerol, and Hepes. Just before use, add DTT, reduced glutathione, and protease inhibitors where indicated, then pass through 0.2 um filter. 100 mM IPTG stock solution (MW=238.3) 595 mg IPTG 25 ml water
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