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文献和实验Method for Single-Cell Electroporation
hrs after electroporation. This usually clears up by 48 hrs. Propidium iodide staining indicated that electroporation does not cause an increase in cell death. For good charge conduction: - Ensure that the specimen remains moist - The platinum
Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
is observed. However, this will need to be determined on a cell type specific basis. 4) miRNA Transfection via Nucleofection and Target Selection A. Grow / expand Target ID Library cells to attain 2 x 107 cells, and trypsinize
effector cell concentration to 4 x 106 cells / ml (for commonly used E:T of 20:1), aliquot 50 µl into each well (triplicates), After 1 hour incubation, spin target cells at 1000 rpm for 5 min. Decant supernatant, resuspend
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