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文献和实验Reverse Transcriptase-PCR Analysis of Gene Expression in Hematopoietic Stem Cells
with anti c-kit MAb and the lineage-negative cells from human bone marrow are incubated with anti-CD34 and anti-CD38 MAb. With a starting population of 2–4 � 10e8 unfractionated mouse bone-marrow cells, a typical isolation procedure yields approx 2 � 10e4 FR
Biological Network Inference and Analysis Using SEBINI and CABIN
factors. Thus, the combined SEBINI–CABIN toolkit aids in the more accurate reconstruction of biological networks, with less effort, in less time. A demonstration web site for SEBINI can be accessed from https://www.emsl.pnl.gov/SEBINI
A Yeast Recombination-Based Cloning System to Produce Chimeric HIV-1 Viruses and Express HIV-1 Genes
technique to insert env sequences first into a yeast cloning vector and then into the common pNL4-3 virus backbone. This technique is not limited to the env gene, but can be used to build chimeric viruses with any HIV-1 gene or genetic element. This cloning
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