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文献和实验functions are very sensitive to changes in pH and hence, buffers are used to stabilize the pH. A pH meter is an instrument that measures the potential difference between a reference electrode and a glass electrode, often combined into one combination
Solutions for Southern Hybridization and Development Hybridization
; sol'n remains turbid Store at 4° C. Add NaAzide for long term storage. Buffer #3: 100mM Tris-Cl, pH 9.5 100mM NaCl 50mM MgCl2 Antibody: 10 µl anti-digoxigenin-AP Fab fragments (Boehringer-Mann cat#1093274) 40ml of Buffer #1 10
Protocol for Annealing Oligonucleotides
Protocol for Annealing OligonucleotidesOligo Name:Lot Number:Total nmol:Volume of Annealing Buffer added:Oligo Name:Lot Number:Total nmol:Volume of Annealing Buffer added:Annealing Buffer: 10mM Tris, pH 7.5 - 8.0, 50mM NaCl, 1mM EDTA1xTE Buffer: 10
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