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文献和实验Reprogramming Fibroblasts with the CytoTune-iPS Reprogramming Kit
9. GlutaMAX™-I Supplement 10. Basic FGF, Recombinant Human 11. β-Mercaptoethanol, 1000X 12. Penicillin-Streptomycin, Liquid 13. Attachment Factor 14. TrypLE™ Select Cell Dissociation Reagent or 0.05% Trypsin/EDTA
Dynabeads® mRNA DIRECT Micro Kit - mRNA isolation for RT-PCR amplification
实验原理 Use the Dynabeads® mRNA DIRECT™ Micro Kit for simple and rapid isolation of mRNA from small cell and tissue samples, for direct use in reverse transcription PCR. The direct mRNA isolation is completed in only 15
Northern Blotting方法(Howell Lab)
Briefly spin up/down in the microfuge. Following the protocol from the Amersham Multiprime Kit (RPN.1601): Add 10 µl of Buffer (solution 1, blue cap vial) Add 5 µl of Primer (solution 2, black cap vial) Add 5 µl of [a-32P]dCTP (3000
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