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文献和实验in step 4.2. Connect the male luer to a 1/8" female luer adaptor. Attach the female luer to a new 18" segment soft tubing. 4) Drill three holes into a 250 ml glass beaker cap in order to fit tubing. Insert a 1.5" segment of soft tubing
around the niche cells. The cap cells cluster in females and hub cells in males act as a niche to control GSC behavior. With highly sophisticated genetic techniques in Drosophila , tremendous progress has been made in understanding the interactions between stem
Culturing Mouse Embryonic Fibroblasts
or PBS to wash instruments between embryos. Aliquot sterile PBS into P60 dishes (2 - 3 per embryo). Euthanize the pregnant female according to standard protocols. CO2 inhalation should be avoided because this induces acidosis.
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