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文献和实验3′‐Modified Oligonucleotides and their Conjugates
) Automated DNA synthesizer and reagents (e.g., PE Biosystems) 1.5‐mL plastic screw‐cap tubes 55°C water
DNA Purification-Modified CTAB Procedure
This protocol originated in Murray and Thompson (1980) and then was modified by Richard Jorgensen and finally published in Wagner et. al 1987. 1.Start with about 10 grams fresh needles. 2.Chop into short pieces. 3.Homogenize, with Polytron
Preparation of 2′‐Deoxy‐2′‐Methylseleno‐Modified Phosphoramidites and RNA
Basic Protocol 3: Deprotection, Purification, and Analysis of RNA Oligonucleotides Containing 2′‐Methylseleno Labels Basic Protocol 4: Enzymatic Ligation of Selenium‐Modified RNA Oligonucleotides Using T4 RNA Ligase
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