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文献和实验Using Cell‐ID 1.4 with R for Microscope‐Based Cytometry
of yeast and mammalian cells processed by Cell‐ID. (A ) From left to right: yeast in focus, slightly defocused (note the dark ring on the border of the cells), and the same cells after Cell‐ID has identified each one and traced their borders correctly
Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
3) Puromycin, G418, and Ganciclovir Kill Curves A. Perform a kill curve for ganciclovir with cells stably expressing the Target ID Library and with puromycin or G418 for wild type cells. B. Plate 1.6 x 104 cells
E. Z. N.A.TM X-press Plasmid Protocol
: It is critical to fully resuspend the cell pellet to obtain optimized DNA yield. 6. Incubate at room temperature for 3-5 minutes. The cell lysate should be nonviscous and slightly cloudy after incubation. 7. Transfer the entire cell lysate into a X
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