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文献和实验Wholemount in situ hybridisation
are carried out in 4mL round-bottom polycarbonate snap-cap tubes unless otherwise stated. Enough liquid must be used to ensure that all embryos are completely covered during each step. All steps are carried out with sufficient rocking to agitate
due to the presence of double cos sites. pop2136 strain (Methods in Enzymology vol.152 pp173-180, 1987), for no apparent reason, can maintain pFOS1 (and other double-cos cosmid vectors) with some stability. The bireplicon is driven by the pUC
Studies of the Ubiquitin Proteasome System
(see recipe ), 4°C Purified native E2 (see Alternate Protocols protocol 3812 and protocol 3913 ; for detection by Coomassie blue staining or immunoblotting), E2 fusion with GST, His or other tag (Wu et al
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