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文献和实验5030) CO2 cell culture incubator 50 ml Nunc/Falcon tubes 15 ml Nunc/Falcon tubes KCl (0.075 M, 0.055% ?) Fixative (methanol/acetic acid 3 : 1) glass microscopy slides Amounts per 5 ml blood: 40 ml RPMI 1640 Medium 10 ml FCS (20%)
was performed in a glass 100-ml beaker as follows. At intensive stirring, 20 drops of NaOH solution (5 M) were added to AgNO3 solution (500 mg) in Milli-Q water (60 ml), and dark brown precipitate formed immediately. Approximately 2 ml of 30% NH4 OH solution
. no. 343775) Lid for reaction tubes: rubber sealing from 1-ml syringes (Dispomed, cat. no. 22009) 12-well plates (Corning Costar, 3513) 18-mm glass coverslips (Marienfeld Superior, cat. no. 0111580) Plate centrifuge
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