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文献和实验. This unit describes protocols to isolate and characterize potential cancer stem cells from a solid tumor. These involve creating a single?cell suspension from tumor tissue, tagging the cell subpopulations of interest, and sorting them into different
Chemically competent XL1‐Blue bacterial cells (recA1 endA1 gyrA96 thi‐1 hsdR17 supE44 relA1 lac [F′ proAB lacIq lacZDM15 Tn10 (TetR)]; Stratagene, cat no. 200249) LB medium powder
Streaking Bacteria for Single Colonies
1. Initial inoculum: - From solid media, touch an isolated colonies with a sterile applicator or toothpick. - From liquid media, touch the culture with the end of a sterile applicator. - From a frozen culture, scrape
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