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上海希言科学仪器有限公司
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文献和实验Purification of Myosin Light Chain Kinase
RPM (4℃).Filter the supernatant through glass wool and discard the pellet (or save for light chain prep).Save 1 ml of supernatant (4℃)for assay.Add pepstatin and TLCK to final concentrations of 1 μg/ml and 40 Og/ml,respectively. Volume of supernatant
miRNA microarrays(McManus Lab)(芯片)
Diamond-tipped glass etching pen (VWR 52865-005) StrataLinker for UV cross-linking Protocol a) Pour 100 ml 0.5x SSC into hydration tray and warm on slide warmer. Set the warmer to 37℃ b) Pre-heat a heating block at max (>100
8). These should beturned on before and after PCR setup for at least 30 min. UVradiation can also be used to remove contaminations fromPCR Master Mixes for use in the PCR. Studies have shownthat exposure to UV light for a period of 30 min can inactivatemany DNA species
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