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文献和实验Growth, Maintenance and Transfection of Suspension Adapted 293-EBNA cells
of plasmids containing the viral EBV origin of replication. This protocol describes the instructions for the growth, maintenance and transfection of suspension adapted 293-EBNA cell line using the PEI expression system. Cells can be purchased from ATCC
Nucleoside Analog Labeling of Neural Stem Cells and Their Progeny
Nucleoside analog pulse labeling is an important technique which can assess the birthdate, cell cycle maintenance, or cycling rates of cells during development. This method has evolved over several decades of use and is now applied
nuclei, a cycling cell component that allows assessment of proper function of doublet discrimination module (DDM) or pulse processing. Please follow the instructions included in the kit for quality control and set-up of the machine. Acquire data
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