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文献和实验Microinjection DNA Purification
We have found the UltraClean™ GelSpin™ Kit (available from Mo Bio Laboratories, Catalog number 12400-250) is a simple and fast way to obtain microinjection quality DNA. This is not to exclude other methods of DNA purification but to say
The Cell Environment (including types of culture medium)
free (Prod. No. W3500, water, tissue culture grade). In most cases water prepared by reverse osmosis and resin cartridge purification with a final resistance of 16-18Mx is suitable. Once prepared the media should be filter sterilized before use
The Cell Environment (including types of culture medium)
culture grade). In most cases water prepared by reverse osmosis and resin cartridge purification with a final resistance of 16-18Mx is suitable. Once prepared the media should be filter sterilized before use. Obviously purchasing 1x liquid media direct
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