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文献和实验Nucleobond Column BAC DNA Purification for Transgenic Mouse Production
, there are a number of factors that could adversely affect plasmid yield, in the case of low copy number plasmids, incomplete bacterial lysis is the number one culprit. The primary reason for this is that in order to get the maximum yield for a particular cartridge
Genomic DNA Extraction - PureLink
3. Centrifuge the cartridge at 12,000 x g for 30 seconds at room temperature. 4. Discard the collection tube and place the spin cartridge into a clean Wash Tube supplied with the kit. 5. Proceed to Washing DNA. Washing DNA Add 500
Purification of Endotoxin Free mAbs
in your preparation (ie. approx. purity on gel x OD280/1.4) and use 1ml of packed gel for every 20mg of antibody. If you use excess the eluted antibody will be more dilute. Prepare Fast Flow S-Sepharose: if this is from a fresh bottle it is usually sufficient
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