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文献和实验Nucleobond Column BAC DNA Purification for Transgenic Mouse Production
, there are a number of factors that could adversely affect plasmid yield, in the case of low copy number plasmids, incomplete bacterial lysis is the number one culprit. The primary reason for this is that in order to get the maximum yield for a particular cartridge
Genomic DNA Extraction - PureLink
. The lysate is mixed with Binding Buffer (L3) and ethanol to adjust conditions for subsequent DNA binding to the PureLink™ Spin Column. The DNA binds to the silica-based membrane in the cartridge and impurities are removed by thorough washing
Purification of Endotoxin Free mAbs
aggregated material, and store frozen at -20 deg C. If your supernant was produced in a hollow fibre cartridge with good yields (ie more than 1mg/ml with less than 5% FCS) then you should expect that more than 90% of the protein visible on a native gel
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