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上海希言科学仪器有限公司
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文献和实验Steady State ATPase Assays Coupled Enzyme System
. Convert to µM by dividing the change in OD340 /min by 6.22 x 10-3 /µM for NADH. Then divide by the motor concentration in µM. Divide by 60 s/min to convert to kcat (s-1 ). The control without motor or MTs is the background
Chick Chorioallantoic Membrane (CAM) Assay
for gross evaluation. For injection, borosilicate glass capillaries (OD 1.0 mm, ID 0.75 mm; Sutter Instrument Company, Novato, CA) were prepared with P-87 micropipette puller (Sutter Instrument Company). Needles were connected with Tygon tubing (ID 1/32
Ab concentrations.OD (Absorbance at 280 nm)/1.35 = X mg/ml.Store Abs at -20℃ or further concentrate by using Centriprep. And store at -20℃.Regenerate Ab column with Regenerate buffer (~200 ml).
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