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文献和实验Sucrose Fractionation of Castor Bean
) with swinging bucket rotor Spectrophotometer and tubes Hemacytometer and phase contrast microscope Procedure 1.Germinate castor beans by soaking overnight, followed by germination in moist vermiculite, or paper towels, at 30° C. Castor beans
Preparation of DNA Template For Direct Sequencing of Large Insert PAC and BAC Plasmid
is complete, about 5-20 minutes. 8. Remove lysate filtrate collection block from manifold. Add 2ul of glycogen type II (Sigma Inc.) at 1ug/ul and ice-cold isopropanol (0.7x volume of filtrate) to each well. Seal with plastic sealing tape and mix. Incubate
Lipoprotein Isolation - First week
of a tissue. 14. Place the metal cap of the tubes, and seal with the heat sealer. As soon as the cap reaches the shoulder of the tube, quicly move the tube to the right and push the cap down with the heat sink. 15. Place the four tubes in the VTi
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