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文献和实验Removal of 32P-ATP from Oligonucleoi
with 0.02% Sodium azide. 2. Plug a 1 mL (blue) pipet tip with Siliconized glass wool. Add 500 ml of 50:50 DE-52 slurry. Pack into a 250 mL column by gently forcing the fluid through with a P-1000 pipetter. 3. Rinse column twice with 1 mL TE
Sephadex G-50 spun column purification
. 1) Plug a 1ml syringe barrel with siliconized glass wool and fill to the top with a sterile suspension of Sephadex G50 equilibrated with H2 O. 2) Once filled place the syringe barrel into a 10ml centrifuge tube and spin at 3,000g
Removal of 32P-ATP from Oligonucleoi
1. Prepare Whatman DE-52 according to manufacturers specifications. Equilibrate at store with 0.02% Sodium azide. 2. Plug a 1 mL (blue) pipet tip with Siliconized glass wool. Add 500 ml of 50:50 DE-52 slurry. Pack into a 250 mL column by gently
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