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文献和实验(as air vent) into one hole and the free ends of the hard and soft tubing through the other 2 holes in the cap into the 250 ml glass bottle (as reservoir) (Fig. 2). Ensure that the hard tubing reaches the bottom of the reservoir (as outflow tubing
off with 0.05% Tween 20 in PBS. 7. Block for about 1 hr with 5% BSA/Tween 20, or overnight with 3% BSA/Tween 20. Reagents and Buffers for Westerns Reagent 30% acrylamide/bisacrylamide (29:1 mix) 100ml(dark bottle at 4°C) 29g acrlamid 1g bisacrylamid
of gel or urea is left. Stand the gel in the lower buffer reservoir so that the notched glass plate faces the top reservoir (make sure the seal is in it’s groove) and the metal plate in the back. Clamp the gel with the metal plate
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