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文献和实验Synthesis and Application of Highly Reactive Amino Linkers for Functional Oligonucleotides
by using the hydrophobic interaction of the monomethoxytrityl group with a reverse?phase resin. Second, the introduction of the carbamate linkage reduces the pK a value of the neighboring primary amine, resulting in an increase in the conjugation yields
In Situ Hybridization Protocols
minutes. Wash twice in DEPC-PBS at room temperature for 5 minutes. Treat slides with 50ug/ml Proteinase K in PK buffer at room temperature for between 8-15 minutes depending on the age of the embryo. Wash once in DEPC-PBS at room
Anderson Lab In Situ Hybridization Protocols
in 4% paraformaldehyde in DEPC-PBS at room temperature for 20 minutes. Wash twice in DEPC-PBS at room temperature for 5 minutes. Treat slides with 50ug/ml Proteinase K in PK buffer at room temperature for between 8-15 minutes depending on the age
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