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文献和实验from the reservoir). 5) Sterilize the above parts by steam autoclaving at 121 °C for 60 min. Additionally autoclave one complete flow chamber, 3 x 2" segments of soft tubing, 1 male and 1 female 1/8" luer adaptor, 4 x ½" and 6 x 5/16" 8-32 flat head
Immunofluorescent Staining of Drosophila Larval Brain Tissue
PBSTX ProLong Gold antifade mounting medium (Invitrogen) Schneider’s insect medium (Sigma-Aldrich) (prechilled) Equipment Coverslips (22- x 22-mm [#1 thickness] and 24- x 40-mm) Dissection dishes Forceps (fine-tipped
Immunofluorescent Staining of Drosophila Larval Brain Tissue
PBSTX ProLong Gold antifade mounting medium (Invitrogen) Schneider’s insect medium (Sigma-Aldrich) (prechilled) Equipment Coverslips (22- x 22-mm [#1 thickness] and 24- x 40-mm) Dissection dishes Forceps (fine-tipped
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