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文献和实验on the stage, cover slip up, of course. Use dark field microscopy to find the focal plane at 40x using the Vaseline film or an air bubble as a target. Switch to 100x, still in dark field mode, and you should be able to focus on high contrast objects
on the stage, cover slip up, of course. Use dark field microscopy to find the focal plane at 40x using the Vaseline film or an air bubble as a target. Switch to 100x, still in dark field mode, and you should be able to focus on high contrast objects
Biotin labeling and hybridization of probe to chromosomes
) 20 µl 10 µl of the hybridization mix is used for one slide and covered with a '18 x 18' cover slip. Care should be taken that the sloution is well mixed, because the 50 % dextran
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