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文献和实验on the stage, cover slip up, of course. Use dark field microscopy to find the focal plane at 40x using the Vaseline film or an air bubble as a target. Switch to 100x, still in dark field mode, and you should be able to focus on high contrast objects
Biotin labeling and hybridization of probe to chromosomes
) 20 µl 10 µl of the hybridization mix is used for one slide and covered with a '18 x 18' cover slip. Care should be taken that the sloution is well mixed, because the 50 % dextran
Differential Interference Contrast (Nomarski, DIC, Hoffman Modulation Contrast)
, using 400x in phase contrast mode. Can you see one or more flagella? Add a drop or two of Lugol's iodine to what remains in the culture tube (ratio should be 4:1 culture to iodine). Mix and prepare a slide of one drop of culture, with cover slip
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