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文献和实验Prehybridization and probe labeling
to each column and centrifuge for 4 minutes at 1,500 RPM. Probe purification Add 185 µL of TE to each probe. Cut the cap off the tube and withdraw all of the probe solution from the tube with a pipet. Place an empty tube
Protein Modification to Probe Intradynein Interactions and In Vivo Redox State
microtubules. In this chapter, we describe how to purify the outer dynein arm from flagella of the unicellular green alga Chlamydomonas , which is one of the best characterized members of this motor class. We also detail the methods that we use to identify
Screening Colonies by Hybridization with Radiolabeled Probe
solution per bottle (42°C with formamide, 68°C without formamide). Prehybridize for 1 - 2 hours. Boil probe for 5 minutes. Make sure the cap of the tube is secured to avoid radioactive spill when tube pops open due to heat expansion! Spin
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