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文献和实验Blue Native Gel Electrophoresis
Store at 4°C 75% (w/v) Glycerol Store at 4°C 10 x Cathode buffer 0.5 M Tricine, 150 mM BisTris No need to adjust pH Store at 4°C 5 x Anode buffer 0.25 M BisTris-HCl, pH 7.0 Adjust pH to 7.0 with HCl at 4°C Store at 4°C 2 x BisTrisACA 200 mM
Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans
-818.2009.03142.x (2009). 6. Hutson, M. S., & Ma, X. Plasma and cavitation dynamics during pulsed laser microsurgery in vivo . Physical review letters. 99, 158104 (2007). 7. Venugopalan, V., Guerra, A., 3rd, Nahen, K., & Vogel
AFLP: not only for fingerprinting, but for positional cloning
) 1.2 µL 25 mM MgCl2 0.08 µL (0.4 U) Taq Pol (Promega) 13.82 µL dH20 ‡ The basic MseI-primer is: 5'-GAT GAG TCC TGA GTA Axy z-3' like with the EcoRI-primer, x, y, and z represent selective bases that are specific and constant
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